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Promega pcineo mammalian expression vector
Pcineo Mammalian Expression Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcineo+expression+vector/pcineo+vector/pmc02978134-160-6-10
Average 90 stars, based on 1 article reviews
pcineo mammalian expression vector - by Bioz Stars, 2026-09
90/100 stars

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Sequencing:

Article Title: G protein–coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1
Article Snippet: Plasmids with full length cDNA for the GPCRs β1AR, β2AR, β3AR and MC4R were obtained from the cDNA Resource Center (cdna.org), with 3xhemagglutinin (3xHA) N-terminal tags in the pcDNA3.1+ expression vector. .. The full length cDNA sequence for KCNJ13 (AJ006128.1) was cloned into the pciNEO expression vector (Promega) with a C terminal 3XFLAG tag (Sigma). ..

Clone Assay:

Article Title: G protein–coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1
Article Snippet: Plasmids with full length cDNA for the GPCRs β1AR, β2AR, β3AR and MC4R were obtained from the cDNA Resource Center (cdna.org), with 3xhemagglutinin (3xHA) N-terminal tags in the pcDNA3.1+ expression vector. .. The full length cDNA sequence for KCNJ13 (AJ006128.1) was cloned into the pciNEO expression vector (Promega) with a C terminal 3XFLAG tag (Sigma). ..

Article Title: Interaction between ROR1 and MuSK activation complex in myogenic cells.
Article Snippet: The ROR family of receptor tyrosine kinases, ROR1 and ROR2, is known to play an important role during skeletal muscle regeneration.. ROR1 has a critical role in regulating satellite cell (SC) proliferation during muscle regeneration, and pro-inflammatory cytokines such as TNF- and IL-1 can induce expression of ROR1 in myogenic cells via NF-κB activation.. While searching for ROR1interacting proteins in myogenic cells, we identified MuSK as a ROR1 binding protein.

Article Title: Decreased accumulation of subgenomic RNA in human cells infected with vaccine candidate DEN4Δ30 increases viral susceptibility to type I interferon.
Article Snippet: Article history: Received 1 February 2018 Received in revised form 19 April 2018 Accepted 20 April 2018 Available online xxxx

Expressing:

Article Title: G protein–coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1
Article Snippet: Plasmids with full length cDNA for the GPCRs β1AR, β2AR, β3AR and MC4R were obtained from the cDNA Resource Center (cdna.org), with 3xhemagglutinin (3xHA) N-terminal tags in the pcDNA3.1+ expression vector. .. The full length cDNA sequence for KCNJ13 (AJ006128.1) was cloned into the pciNEO expression vector (Promega) with a C terminal 3XFLAG tag (Sigma). ..

Article Title: Interaction between ROR1 and MuSK activation complex in myogenic cells.
Article Snippet: The ROR family of receptor tyrosine kinases, ROR1 and ROR2, is known to play an important role during skeletal muscle regeneration.. ROR1 has a critical role in regulating satellite cell (SC) proliferation during muscle regeneration, and pro-inflammatory cytokines such as TNF- and IL-1 can induce expression of ROR1 in myogenic cells via NF-κB activation.. While searching for ROR1interacting proteins in myogenic cells, we identified MuSK as a ROR1 binding protein.

Article Title: The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha
Article Snippet: .. The inserts were subcloned into the pCIneo expression vector (Promega; Madison, WI). ..

Article Title: EMID1: a multifunctional molecule identified in a murine model for the invasion- independent metastasis pathway
Article Snippet: .. The construct pCIneo − Emid1 was made by subcloning this cDNA into the pCIneo expression vector (Promega). .. 66 − 4 cells were transfected with the pCIneo − Emid1 vector using Lipofectamine 3000 (Thermo Fisher Scienti c).

Article Title: Characterization of the Mel1c melatoninergic receptor in platypus ( Ornithorhynchus anatinus )
Article Snippet: .. Coding sequences for the platypus (XM 001512887), X . laevis (U67880), and G . gallus (NM 205361.1) Mel1c melatonin receptors were synthesized by Genecust (Dudelange, Luxemburg) and subcloned into the pCIneo expression vector (Promega Madison, Wisconsin, USA). .. COS7 cells obtained from the American Type Culture Collection were maintained in DMEM GlutaMAX (Thermo Fisher Scientific, Waltham, USA) supplemented with 10% (v/v) fetal calf serum.

Article Title: Decreased accumulation of subgenomic RNA in human cells infected with vaccine candidate DEN4Δ30 increases viral susceptibility to type I interferon.
Article Snippet: Article history: Received 1 February 2018 Received in revised form 19 April 2018 Accepted 20 April 2018 Available online xxxx

Article Title: The expression, localisation and interactome of pigeon CRY2
Article Snippet: .. GFP-tagged recombinant clCRYs were expressed in primary cell cultures of pigeon embryonic fibroblasts (PEF) using the pCIneo expression vector (Promega, E1841). .. PEFs were cultured at 37 °C on sterile, uncoated 10-cm cell culture dishes (0030702115, Eppendorf).

Plasmid Preparation:

Article Title: G protein–coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1
Article Snippet: Plasmids with full length cDNA for the GPCRs β1AR, β2AR, β3AR and MC4R were obtained from the cDNA Resource Center (cdna.org), with 3xhemagglutinin (3xHA) N-terminal tags in the pcDNA3.1+ expression vector. .. The full length cDNA sequence for KCNJ13 (AJ006128.1) was cloned into the pciNEO expression vector (Promega) with a C terminal 3XFLAG tag (Sigma). ..

Article Title: Interaction between ROR1 and MuSK activation complex in myogenic cells.
Article Snippet: The ROR family of receptor tyrosine kinases, ROR1 and ROR2, is known to play an important role during skeletal muscle regeneration.. ROR1 has a critical role in regulating satellite cell (SC) proliferation during muscle regeneration, and pro-inflammatory cytokines such as TNF- and IL-1 can induce expression of ROR1 in myogenic cells via NF-κB activation.. While searching for ROR1interacting proteins in myogenic cells, we identified MuSK as a ROR1 binding protein.

Article Title: The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha
Article Snippet: .. The inserts were subcloned into the pCIneo expression vector (Promega; Madison, WI). ..

Article Title: EMID1: a multifunctional molecule identified in a murine model for the invasion- independent metastasis pathway
Article Snippet: .. The construct pCIneo − Emid1 was made by subcloning this cDNA into the pCIneo expression vector (Promega). .. 66 − 4 cells were transfected with the pCIneo − Emid1 vector using Lipofectamine 3000 (Thermo Fisher Scienti c).

Article Title: Characterization of the Mel1c melatoninergic receptor in platypus ( Ornithorhynchus anatinus )
Article Snippet: .. Coding sequences for the platypus (XM 001512887), X . laevis (U67880), and G . gallus (NM 205361.1) Mel1c melatonin receptors were synthesized by Genecust (Dudelange, Luxemburg) and subcloned into the pCIneo expression vector (Promega Madison, Wisconsin, USA). .. COS7 cells obtained from the American Type Culture Collection were maintained in DMEM GlutaMAX (Thermo Fisher Scientific, Waltham, USA) supplemented with 10% (v/v) fetal calf serum.

Article Title: Decreased accumulation of subgenomic RNA in human cells infected with vaccine candidate DEN4Δ30 increases viral susceptibility to type I interferon.
Article Snippet: Article history: Received 1 February 2018 Received in revised form 19 April 2018 Accepted 20 April 2018 Available online xxxx

Construct:

Article Title: EMID1: a multifunctional molecule identified in a murine model for the invasion- independent metastasis pathway
Article Snippet: .. The construct pCIneo − Emid1 was made by subcloning this cDNA into the pCIneo expression vector (Promega). .. 66 − 4 cells were transfected with the pCIneo − Emid1 vector using Lipofectamine 3000 (Thermo Fisher Scienti c).

Subcloning:

Article Title: EMID1: a multifunctional molecule identified in a murine model for the invasion- independent metastasis pathway
Article Snippet: .. The construct pCIneo − Emid1 was made by subcloning this cDNA into the pCIneo expression vector (Promega). .. 66 − 4 cells were transfected with the pCIneo − Emid1 vector using Lipofectamine 3000 (Thermo Fisher Scienti c).

Synthesized:

Article Title: Characterization of the Mel1c melatoninergic receptor in platypus ( Ornithorhynchus anatinus )
Article Snippet: .. Coding sequences for the platypus (XM 001512887), X . laevis (U67880), and G . gallus (NM 205361.1) Mel1c melatonin receptors were synthesized by Genecust (Dudelange, Luxemburg) and subcloned into the pCIneo expression vector (Promega Madison, Wisconsin, USA). .. COS7 cells obtained from the American Type Culture Collection were maintained in DMEM GlutaMAX (Thermo Fisher Scientific, Waltham, USA) supplemented with 10% (v/v) fetal calf serum.

Polymerase Chain Reaction:

Article Title: Decitabine-induced DNA methylation-mediated transcriptomic reprogramming in human breast cancer cell lines; the impact of DCK overexpression
Article Snippet: .. The resulting PCR product with the ORF of the human DCK gene was then subcloned into the pCIneo expression vector (Promega Corporation, Madison, WI) named as pCIneoDCK. ..

Recombinant:

Article Title: The expression, localisation and interactome of pigeon CRY2
Article Snippet: .. GFP-tagged recombinant clCRYs were expressed in primary cell cultures of pigeon embryonic fibroblasts (PEF) using the pCIneo expression vector (Promega, E1841). .. PEFs were cultured at 37 °C on sterile, uncoated 10-cm cell culture dishes (0030702115, Eppendorf).



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